Table 1 Sequences of primers used for this study
Primer | Length | Sequence (5′ → 3′) | Purpose |
|---|---|---|---|
pBBR1MCS-2 vector | |||
XhoI GFPmut2 F1 | 60 | GGCGGCCTCGAGAGGAGGATCTATTCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTG | Cloning GFPmut2 |
XbaI GFPmut2 R1 | 51 | GCCGCCTCTAGACTATTTGTATAGTTCATCCATGCCATGTGTAATCCCAGC | |
BamHI RBS AdhE2824 F1 | 68 | GCCGCGGATCCAGGAGGATCTATTCATGAAAGTTACAAATCAAAAAGAACTAAAACAAAAGCTAAATG | Cloning adhE2 824 |
SacI 6X His AdhE2824 R1 | 62 | CACCCGGAGCTCTAAGTGGTGATGGTGATGATGAAATGATTTTATATAGATATCCTTAAGTT | |
SacI AdhE2824 R1 | 43 | CACCCGGAGCTCTAAAATGATTTTATATAGATATCCTTAAGTT | |
XhoI RBS AdhEBisB18 F1 | 57 | GACGACCTCGAGAGGAGGATCTATTCGTGACCTTATCTACCCCGTCCGACCTCGACA | Cloning adhE BisB18 |
XbaI 6X His AdhEBisB18 R1 | 60 | CACCCGTCTAGACTAGTGGTGATGGTGATGATGTTCCGCGGCGTTCGCCGTCGCCACCGA | |
XbaI AdhEBisB18 R1 | 52 | CAGTCACCCGTCTAGACTATTCCGCGGCGTTCGCCGTCGCCACCGACAATGT | |
pMG105P vector | |||
XbaI GFPmut2 F1 | 60 | GGCGGCTCTAGAAGGAGGATCTATTCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTG | Cloning GFPmut2 |
SalI GFPmut2 R1 | 53 | GGCGGCCGGTCGACCTATTTGTATAGTTCATCCATGCCATGTGTAATCCCAGC | |