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Table 1 Saccharide composition of onion epidermal wall by met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

 

µg/mg dry weight

%

Trial 1

Trial 2

Trial 3

Mean

Total

mTFA

Sae

mTFA

Sae

mTFA

Sae

mTFA

Sae

Glc

6.9

21.6

13.1

144.9

17.1

186.3

12.3 (2.3)

117.6 (19.8)

130.0

24

GalA

183.5

16.5

255.5

8.5

321.3

29.0

253.4 (23.1)

18.0 (2.4)

271.5

51

Gal

64.4

1.9

52.0

2.8

87.0

4.5

67.8 (7.2)

3.0 (0.6)

70.9

13

Xyl/Man*

27.8

6.7

21.7

7.6

29.5

8.8

26.3 (1.8)

7.7 (0.4)

34.0

6

Rha

14.8

0.5

5.4

1.0

12.9

1.0

11.0 (1.9)

0.8 (0.1)

11.9

2

Ara

8.0

0.2

5.1

1.0

11.1

0.8

8.1 (1.2)

0.6 (0.2)

8.7

2

Fuc

3.1

0.3

2.4

1.5

4.1

1.6

3.2 (0.4)

1.1 (0.4)

4.4

1

GlcA

0.8

0.0

0.0

0.0

1.8

0.0

0.9 (0.3)

0.0 (0.0)

0.9

0.2

Total

309.3

47.7

355.1

167.3

485.0

232.0

383.1

149.0

532.1

100

  1. mTFA, peel hydrolyzed with methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman’s hydrolysis) on residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”. (SEM), standard error of mean of 3 biological replicates