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Table 2 Saccharide composition of onion epidermal wall pre-extracted with chloroform and met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

 

µg/mg dry weight

%

Trial 1

Trial 2

Trial 3

mean

Total

Chl + mTFA

Sae

Chl + mTFA

Sae

Chl + mTFA

Sae

Chl + mTFA

Sae

Glc

26.15

360.28

7.57

151.44

20.2

172.6

18.0 (4.5)

228.1 (54.2)

246.1

34

GalA

422.66

16.00

144.28

65.46

320.4

22.1

295.8 (66.4)

34.5 (12.7)

330.3

45

Gal

101.77

5.01

4.42

4.84

114.0

4.3

73.4 (28.3)

4.7 (0.2)

78.1

11

Xyl/Man*

44.27

17.05

4.57

11.80

38.0

8.7

29.0 (10.1)

12.5 (2.0)

41.5

6

Rha

15.49

0.67

1.98

0.76

14.4

0.7

10.6 (3.5)

0.7 (0.02)

11.3

2

Ara

15.78

0.67

0.83

0.72

11.3

0.7

9.3 (3.6)

0.7 (0.02)

10.0

1

Fuc

4.97

6.72

3.81

0.00

4.4

0.0

4.4 (0.3)

2.2 (1.8)

6.6

1

GlcA

1.9

0.0

0.0

0.0

5.0

0.0

2.3 (1.3)

0.0 (0.0)

2.3

0.3

Total

633.0

406.4

167.5

235.0

527.8

209.1

442.8

283.5

726.3

100

  1. Chl + mTFA, peel chloroform pretreated followed by methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman's hydrolysis) on chloroform pretreated residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”; (SEM), standard error of mean of 3 biological replicates