Fig. 4
From: Systematic approaches to C-lignin engineering in Medicago truncatula
Engineering lignin composition in M. truncatula hairy routes by transformation with MtCOMT-MtCCoAOMT RNAi and ChLAC8 overexpression constructs in the comt mutant background. A Lignin biosynthesis pathway designed for accumulation of C-lignin. The reactions which are blocked during the phase of C-lignin biosynthesis in the Cleome seed coat are shown with a 
sign. B–E Monolignol compositions of transgenic lines determined by thioacidolysis. Data show % of H-lignin monomer (B), C-lignin monomer (C), G-lignin monomer (D) and S-lignin monomer (E). cGUS, GUS control in comt mutant background; RGUS, GUS control in R108 wild-type background. Stem, mature R108 stem. −/−, no transgenic event; −/+, transgenic roots only harboring ChLAC8 overexpression construct; ±, transgenic roots only harboring MtCOMT-MtCCoAOMT RNAi construct; +/+, transgenic roots harboring both MtCOMT-MtCCoAOMT RNAi and ChLAC8 overexpression constructs. CCL, RNAi construct for MtCOMT (C) and MtCCoAOMT (C) and overexpression construct for ChLAC8 (L). Data are means ± SD derived from three biological replicates