Fig. 5
From: Systematic approaches to C-lignin engineering in Medicago truncatula
Monolignol pathway metabolite levels in M. truncatula MtCOMT-MtCCoAOMT RNAi and ChLAC8 overexpression hairy roots. The engineered lines, in the comt mutant background, are a selection of those shown in Fig. 4. The Figure shows metabolite levels superimposed on a scheme of the monolignol pathways. Metabolite levels were determined by LC–MS/MS transitions pre-determined for individual compounds and quantified by comparison to authentic standards. Structures are shown in Fig. 1. CCL, RNAi construct for MtCOMT (C) and MtCCoAOMT (C) and overexpression construct for ChLAC8 (L). c_GUS, GUS control in comt mutant background; R_GUS, GUS control in R108 wild-type background; stem, mature R108 stem (controls, right-hand 4 bars in all panels). −/−, no transgenic event; −/+, transgenic roots only harboring ChLAC8 overexpression construct; ±, transgenic roots only harboring MtCOMT-MtCCoAOMT RNAi construct; +/+, transgenic roots harboring both MtCOMT-MtCCoAOMT RNAi and ChLAC8 overexpression constructs. Data are means ± SD derived from three biological replicates