Fig. 6
From: Systematic approaches to C-lignin engineering in Medicago truncatula
Engineering lignin composition in M. truncatula hairy routes by transformation for SmF5H, MtCOMT-MtCCoAOMT RNAi and ChLAC8-ChLAC15 expression in the comt mutant background. A Lignin biosynthesis pathway designed for accumulation of C-lignin. B–E Monolignol compositions of transgenic lines determined by thioacidolysis. Data show % of H-lignin monomer (B), C-lignin monomer (C), G-lignin monomer (D) and S-lignin monomer (E). c_GUS, GUS control in comt mutant background; R_GUS, GUS control in R108 wild-type background. Stem, mature R108 stem. −/−/−, no transgenic event; ±/−, transgenic roots only harboring SmF5H overexpression construct; -/±, transgenic roots only harboring MtCOMT-MtCCoAOMT RNAi construct; −/−/+, transgenic roots harboring only ChLAC8-ChLAC15 construct; −/+/+, transgenic roots harboring both MtCOMT-MtCCoAOMT RNAi and ChLAC8-ChLAC15 overexpression constructs.. FCCLL, overexpression construct for SmF5H (F), RNAi construct for MtCOMT-MtCCoAOMT RNAi (CC) and overexpression construct for overexpression for ChLAC8-ChLAC15 (LL). Data are means ± SD derived from three biological replicates