Regression modelling of conditional morphogene expression links and quantifies the impact of growth rate, fitness and macromorphology with protein secretion in Aspergillus niger

Table 2 Prediction of protein titres using regression modelling

	P-value	Coefficient	Change relative to MA70.15 control strain (%)	Predicted protein concentration (mg/g)
			0	2.83
Growth rate	0.0041	0.6592	− 10	1.97
			− 20	1.12
			− 30	0.48
Fitness under heat stress	0.0047	10.6824	− 10	2.62
			− 20	1.55
			− 30	0.48
Fitness under cell wall stress	0.0009	− 13.4059	− 10	5.03
			− 20	6.37
			− 30	7.71
Pellet diameter	0.0002	− 0.0020	− 10	4.14
			− 20	4.60
			− 30	5.05

Test variables were only incorporated into the regression model where p < 0.05. Hypothetical decreases (10, 20, 30%) in the indicated strain parameter relative to the MA70.15 control were used to calculate predicted protein titres (mg/g)

ISSN: 2731-3654