Fig. 1

Metabolic strategies for enhancing l-methionine production in E.coli. Glc, glucose; 3-PG, glyceraldehyde-3-phosphate; PEP, phosphoenolpyruvate; PYR, pyruvate; Ac-CoA, acetyl coenzyme A; OAA, oxaloacetate; ASP, l-aspartate; ASP-P, aspartyl-β-phosphate; ASP-SA, aspartyl-β-semialdehyde; HOM, l-homoserine; O-SUCC-HOM, O-succinyl-l-homoserine; CYSTA, l-cystathionine; HOMCYS, l-homocysteine; MET, l-methionine; 3-PHP, 3-Phosphonooxypyruvate; SER-P, 3-phosphoserine; SER, l-serine; O-AC-SER, O-acetyl-l-serine; CYS, l-cysteine; APS, adenosine 5-phosphosulfate; PAPS, 3-phosphoadenosine 5-phosphosulfate; GLY, l-glycine; M-THF, 5-methyltetrahydrofolate; THF, tetrahydrofolate; THR, l-threonine; 2-KB, 2-ketobutyrate; ILE, l-isoleucine; pykFA, pyruvate kinase; metL, fused aspartate kinase/homoserine dehydrogenase 2; metA, l-homoserine O-succinyltransferase; metB, cystathionine γ-synthase; metC, cystathionine β-lyase; metE/H, homocysteine methyltransferase; yjeH, l-methionine exporter; MetD, l-methionine absorption transporter operon; RhtA, l-homoserine exporter; serA, phosphoglycerate dehydrogenase; serC, phosphoserine aminotransferase; serB, phosphoserine phosphatase; cysE, serine O-acetyltransferase; cysK, cysteine synthase; cysN, sulfate adenylyltransferase subunit 1; cysD, sulfate adenylyltransferase subunit 2; cysC, adenylylsulfate kinase; cysH, phosphoadenosine phosphosulfate reductase; cysJ, sulfite reductase (NADPH) flavoprotein alpha-component; cysI, sulfite reductase (NADPH) flavoprotein beta-component