Table 2 Strains used in this study with relevant characteristics

EC135

TOP10 Δdcm::FRT recA⁺∆dam::FRT, genotype: F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZ ΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Str^R) endA1 λ⁻ Δdcm::FRT recA⁺Δdam::FRT

[44]

W3110

F-λ-rph-1 INV(rrnD,rrnE)

[45]

MET1

W3110ΔmetJ

This study

MET2

W3110ΔmetJ metA^fbr

This study

MET3

W3110ΔmetJ metA^fbrΔrhtA

This study

MET4

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr

This study

MET5

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metBL::P_trc

This study

MET6

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trc

This study

MET7

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metF::P_trc

This study

MET8

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC

This study

MET9

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metCΔmetD

This study

MET10

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeH

This study

MET11

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔsucCD

This study

MET12

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔpykA

This study

MET13

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔpykAΔpykF

This study

MET14

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔpykAΔpykFΔrhtA::P₂₃₁₁₉-cysE

This study

MET15

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔpykAΔpykFΔrhtA::P₂₃₁₁₉-cysE^fbr

This study

MET16

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔpykAΔpykFΔrhtA::P₂₃₁₁₉-cysE^fbrΔrhtA::P₂₃₁₀₅-serA^fbr

This study

MET17

W3110ΔmetJ metA^fbrΔrhtA::P_trc-metA^fbr P_metC::P_trcΔrhtA::P_trc-metC ΔmetD::P_L-yjeHΔpykAΔpykFΔrhtA::P₂₃₁₁₉-cysE^fbrΔrhtA::P₂₃₁₀₅-serA^fbrΔygaY::P_trc-cysDN

This study

E. coli W3110/p1

E. coli W3110 carrying pBR322-metA

This study

W3110/p2

E. coli W3110 carrying pBR322-metA^I124L

This study

E. coli W3110/p3

E. coli W3110 carrying pBR322-metA^I229Y

This study

E. coli W3110/p4

E. coli W3110 carrying pBR322-metA^{I124L, I229Y}

This study

E. coli W3110/p5

E. coli W3110 carrying pBR322-metA^{R27C, I296S, P298L}

This study

E. coli W3110/p6

E. coli W3110 carrying pBR322-metA^{R27C, I124L, I296S, P298L}

This study

E. coli W3110/p7

E. coli W3110 carrying pBR322-metA^{R27C, I229Y, I296S, P298L}

This study

E. coli W3110/p8

E. coli W3110 carrying pBR322-metA^{R27C, I124L, I229Y, I296S, P298L}

This study

E. coli W3110/

pBR322

E. coli W3110 carrying pBR322

This study

MET16/p9

MET16 carrying pACYC184-cysD

This study

MET16/p10

MET16 carrying pACYC184-cysN

This study

MET16/p11

MET16 carrying pACYC184-cysDN

This study

MET16/p12

MET16 carrying pACYC184-cysC

This study

MET16/p13

MET16 carrying pACYC184-cysH

This study

MET16/p14

MET16 carrying pACYC184-cysJ

This study

MET16/p15

MET16 carrying pACYC184-cysI

This study

MET16/p16

MET16 carrying pACYC184-cysJI

This study

MET16/

pACYC184

MET16 carrying pACYC184

This study