Fig. 1

Differential transcription of genes in the cip-cel operon initiated by RNA cleavage. a Transcriptional profiles of the cip-cel operon grown on glucose, cellobiose and cellulose. Eleven intergenic regions (IRs) are indicated by their length. b Schematic representation of dual-fluorescence reporter system for analysis of RNA cleavage, where IRs were inserted between fbfp and mcherry. c Northern blotting analysis of transcripts using the dual-fluorescence reporter system carrying each IR with fbfp- and mcherry-targeting probes. Black arrows highlight the positions of bands that corresponded to transcripts, as indicated on the right side of the panel. d Relative transcription level of fbfp and mcherry in artificial operons harboring various IRs, as measured by qRT-PCR. Data were normalized via the transcript level of the gene of Ccel_RS01560, encoding the RNA polymerase beta subunit. The artificial operon harboring no IRs was used as a control. Error bars indicate the standard deviation of the mean from experiments done in triplicate