Fig. 3From: Stem-loop structures control mRNA processing of the cellulosomal cip-cel operon in Ruminiclostridium cellulolyticumFunctional analysis of secondary structures predicted in IRs harboring cleavage sites. The secondary structures of IR1 (a), IR5 (b), IR2 (c), IR7 (d), IR4 (e), and IR10 (f) were, respectively, mutated and inserted into the dual-fluorescence reporter system. Transcripts were analyzed by Northern blotting using fbfp- and mcherry-targeting probes. Black arrows highlight the positions of bands that correspond to transcripts as indicated on the right side of the panel. 16S rRNA was used as a loading controlBack to article page