Table 4 ANOVA for response surface polynomial model

Model

6.67E+05

9

74,136.94

807.7

< 0.0001 significant

X₁-Substrate ratio

2.39E+05

1

2.39E+05

2602.33

< 0.0001

X₂-IC

57,612.09

1

57,612.09

627.67

< 0.0001

X₃-Dilution

10,835.46

1

10,835.46

118.05

< 0.0001

X₁X₂

29,403.13

1

29,403.13

320.34

< 0.0001

X₁X₃

4560.13

1

4560.13

49.68

0.0002

X₂X₃

21.13

1

21.13

0.2302

0.646

X₁²

1.09E+05

1

1.09E+05

1186.38

< 0.0001

X₂²

2.66E+05

1

2.66E+05

2894.89

< 0.0001

X₃²

1927.55

1

1927.55

21

0.0025

Residual

642.51

7

91.79

Lack of fit

627.34

5

125.47

16.55

0.058 not significant

Pure error

15.17

2

7.58

Cor total

6.68E+05

16