A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis

Table 1 The K_m and K_cat values of the mutants to substrate ADP and polyP₆

Substrates	Strains	K_m (mM)	K_cat (S⁻¹)	K_cat/K_m (S⁻¹ M⁻¹)
ADP	WT	1.39 \(\pm 0.\)07	10.04 \(\pm\) 0.13	7.22 \(\times {10}^{3}\)
ADP	K103E	0.81 \(\pm 0.\)12	19.69 \(\pm 0.12\)	24.3 \(\times {10}^{3}\)
ADP	D82N	0.95 \(\pm 0.\)03	15.05 \(\pm 0.04\)	15.8 \(\times {10}^{3}\)
polyP₆	WT	2.3 \(\pm\) 0.02	35.16 \(\pm 0.19\)	15.3 \(\times {10}^{3}\)
polyP₆	K103E	2.24 \(\pm\) 0.02	37.17 \(\pm\) 0.02	\(17.0\times {10}^{3}\)
polyP₆	D82N	2.12 \(\pm\) 0.01	49.24 \(\pm\) 0.13	23.2 \(\times {10}^{3}\)

ISSN: 2731-3654