Fig. 3From: Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic rolesBoth Hill and Michaelis–Menten kinetics of PfkA generated using SigmaPlot® 11.2. (A) Activity of PfkA versus varied F6P concentration fitted to Hill equation predicts a relatively high Km of 1.9 mM for F6P, as well as Vmax, while GTP(2 mM) is used as a phosphate donor, in comparison to when (B) ATP(2 mM) is used fitted to Michaelis–Menten kinetics, producing approximately a third of the velocity, but a reduced Km for F6P. (C) The Km of GTP and (D) ATP fitted to Michaelis–Menten kinetics determined by varying the concentration of the phosphate donors while keeping the concentration of the substrate constant in the presence of NH4+. All assays were performed in triplicateBack to article page