Fig. 5From: Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic rolesThe pH range over which PfkA showed activity with both phosphate donors and F6P as the substrate. In order to reduce confounding effects only sulfonic acid buffering agents with varying pKa were used. The velocity of the enzyme in each reaction was recorded in the presence of 2Â mM of both the substrate and respective phosphate donor. Each assay condition was performed in triplicateBack to article page