Fig. 8From: Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles(A) Potential activating/ inhibiting compounds added to the assays to determine which conditions PfkB prefer as well as any possible feedback effect from other metabolic sugars. The velocity of the enzyme in each reaction was recorded in the presence of 2.24Â mM substrate, 2.24Â mM phosphate donor and 2.24Â mM effector excluding MgCl2 (5.6Â mM). (B) Relative activity of the enzyme with potential activating/inhibiting compounds added to the assays, compared to assays without any potential effectors other than MgCl2(5Â mM) with ATP as the phosphate donor while (C) utilized GTP. The concentration of PPi used in the assay was 0.4Â mM, while the other effectors remained at 2.24Â mM (B, C)Back to article page