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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Regulation of acetate tolerance by small ORF-encoded polypeptides modulating efflux pump specificity in Methylomonas sp. DH-1

Fig. 3

WatR-dependent activation of genes encoding an RND-type efflux pump contributing to organic acid tolerance. A Gene structure and putative functions of watPAB and watO genes regulated by WatR. Side view of the predicted WatABO efflux pump is shown. OM: outer membrane; IM: inner membrane. B WatR-dependent activation of the efflux pump genes. Transcript levels were detected by qRT-PCR in the wild-type, JHM80, and JHM80 ΔwatR (JHM82) strains and indicated as values relative to those of wild type. C The effect of deleting the efflux pump genes on acid tolerance. The JHM80 strain and JHM80 strain lacking the WatABO efflux pump (JHM87) were grown in NMS media with 20% (v/v) methane without or with the indicated weak organic acids. Each value represents the average ± standard deviations from two independent experiments

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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