Fig. 5

WatR-dependent regulation of smORF genes upon acetate stress. A WatR-dependent regulation of smORF genes. Locations of five unannotated smORFs (watS1-watS5) are aligned with the WatR-binding peaks detected via ChIP-Seq and transcript levels detected via RNA-seq using the IGV 2.3.72 program. RNA-seq analysis was performed in the wild-type and ΔwatR (JHM15) strains with or without acetate treatment. B The promoter sequences of watS1-watS5 with their expected -35 box, -10 box, and TSS. The conserved WatR-binding sites are shown as inverted arrows. C The homology alignment of amino acid sequences of WatS1 to WatS5. A putative transmembrane domain region conserved in WatS1 to WatS4 is indicated. D WatR-dependent regulation of WatABO efflux pump genes. The gene locations were aligned with the WatR-binding peaks detected via ChIP-seq analysis and transcript levels detected by RNA-seq. E The promoter sequences of the divergently transcribed watPAB and watO genes with their expected -35 box, -10 box, and TSS. The putative WatR-binding sites are shown as inverted arrows