Table 1 Strains used in this study

Methylomonas sp. DH-1

Wild-type strain

[54]

JHM15

watR operon deletion in DH-1

DH-1 Δ(watR-smtM-rstM)::Kan^R

This study

JHM16

fliE deletion in DH-1

DH-1 ΔfliE::kan^R

This study

JHM161

watS1 overexpression in DH-1

DH-1 ΔfliE:: P_mxaF-watS1-T_rrnB-Kan^R

This study

JHM162

watS2 overexpression in DH-1

DH-1 ΔfliE:: P_mxaF-watS2-T_rrnB-Kan^R

This study

JHM163

watS3 overexpression in DH-1

DH-1 ΔfliE:: P_mxaF-watS3-T_rrnB-Kan^R

This study

JHM164

watS4 overexpression in DH-1

DH-1 ΔfliE:: P_mxaF-watS4-T_rrnB-Kan^R

This study

JHM165

watS5 overexpression in DH-1

DH-1 ΔfliE:: P_mxaF-watS5-T_rrnB-Kan^R

This study

JHM17

watS1 deletion in DH-1

DH-1 ΔwatS1::Kan^R

This study

JHM18

WatABO pump deletion in DH-1

DH-1 Δ(watPAB-watO)::Amp^R

This study

JHM181

fliE deletion in JHM18

JHM18 ΔfliE::Kan^R

This study

JHM182

watS1 deletion in JHM18

JHM18 ΔwatS1::Kan^R

This study

JHM183

watS1 overexpression in JHM18

JHM18 ΔfliE::P_mxaF-watS1-T_rrnB-Kan^R

This study

JHM80

Evolved strain from DH-1

[4]

JHM82

watR operon deletion in JHM80

JHM80 Δ(watR-smtM-rstM)::Kan^R

[4]

JHM87

WatABO pump deletion in JHM80

JHM80 Δ(watPAB-watO)::Kan^R

This study

JHM16WF

watR-Flag overexpression in DH-1

DH-1 ΔfliE::P_EFTu-watR-Flag-T_rrnB-Kan^R

This study

JHM80WF

watR-Flag tagging in JHM80

JHM80 watR-Flag-T_rrnB-Kan^R

This study

JHM161T

watS1-T7 overexpression in DH-1

DH-1 ΔfliE::P_mxaF-watS1- T7-T_rrnB-Kan^R

This study