Table 2 Plasmids used in this study

Plasmids for gene deletion in Methylomonas sp. DH-1

 pDel2-WSR

pDel2-U_watR-[T_rrnB-Kan^R]-D_rstM

[4]

 pDel2-fliE

pDel2-U_fliE-[T_rrnB-Kan^R]-D_fliE

[4]

 pDel2-watABPO(A)

pDel2-D_watO-[T_rrnB-Amp^R]-D_watA

This study

 pDel2-watABPO(K)

pDel2-D_watO-[T_rrnB-Kan^R]-D_watA

This study

 pDel2-watS1

pDel2-U_watS1-[T_rrnB-Kan^R]-D_watS1

This study

Plasmids for gene expression in Methylomonas sp. DH-1

 pFliE-mxaF

pDel2-U_fliE-T_rrnB-[P_mxaF-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-watS1

pFliE-mxaF-U_fliE-T_rrnB -[P_mxaF-watS1-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-watS2

pFliE-mxaF-U_fliE-T_rrnB -[P_mxaF-watS2-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-watS3

pFliE-mxaF-U_fliE-T_rrnB -[P_mxaF-watS3-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-watS4

pFliE-mxaF-U_fliE-T_rrnB -[P_mxaF-watS4-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-watS5

pFliE-mxaF-U_fliE-T_rrnB -[P_mxaF-watS5-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-watS1-T7

pFliE-mxaF-U_fliE-T_rrnB -[P_mxaF-watS1-T7-T_rrnB-Kan^R]-D_fliE

This study

 pFliE-EFTu-watR-Flag

pFliE-mxaF-U_fliE-T_rrnB -[P_EFTu-watR-G4S-Flag-T_rrnB-Kan^R]-D_fliE

This study

 pWatR-G4S-Flag

pDel2-watR-G4S-Flag-[T_rrnB-Kan^R]-D_watR

This study

Plasmids for gene expression in E. coli

 pGEX-4T-1-WatR

pGEX-4T-1-P_tac-GST-watR

This study