Fig. 5

Tyrosine production of chromosome engineered strains GsBR1 to GsBR5. Strains GsBR1 (white), GsBR2 (gray), GsBR3 (orange), GsBR4 (green), and GsBR5 (yellow), in which one to five Tyr-producing modules were integrated at different gene loci on the chromosome, were tested. Strains GsBR1, GsBR3, and GsBR5 transformed with pQE1a-Gs1 (GsPheH1) or pSTV-BH4R (BH4R) were also evaluated. Each strain was cultured for 48 h at 30 °C. Tyr, bars; OD, blue circles. Data are presented as mean values with standard deviations for three independent experiments. Symbols without an error bar indicate that they are larger than the size of the error bar