Table 1 Plasmids used in this study
Plasmids | Description | Sources |
|---|---|---|
pSTV28 | Cloning and protein expression vector; lac promoter, p15A ori, CmR | Takara Bio |
pSTV28N | pSTV28N derivative; NdeI site created downstream of the ribosome binding site | This study |
pSTV-BH4R | pSTV28N derivative; expression of PCD and DHPR genes as an operon | This study |
pQE-80L | Protein expression vector; T5 promoter, lacI, ColE1 ori of pBR322, ApR | Qiagen |
pQE1a-Red | pQE-80 derivative; protein expression vector; tac promoter, lacI, DsRed-monomer gene, ColE1 ori, ApR | This study |
pQE1a | pQE1a-Red derivative; deletion of DsRed-monomer gene as a control plasmid | This study |
pQE1a-RatC | pQE1a-Red derivative; expression of RatPheHc, codon-optimized for E. coli | This study |
pQE1a-Bs | pQE1a-Red derivative; expression of BsPheH gene of Bacillus sp. | This study |
pQE1a-Cn | pQE1a-Red derivative; expression of CnPheH gene of C. necator | This study |
pQE1a-Cv | pQE1a-Red derivative; expression of CvPheH gene of C. violaceum, codon-optimized for E. coli | This study |
pQE1a-Gs1 | pQE1a-Red derivative; expression of GsPheH1 gene of Gulbenkiania sp. | This study |
pQE1a-Gs2 | pQE1a-Red derivative; expression of GsPheH2 gene of Gulbenkiania sp. | This study |
pQE1a-Pp | pQE1a-Red derivative; expression of PpPheH gene of P. putida | This study |
pQE1a-Xo | pQE1a-Red derivative; expression of XoPheH gene of X. oryzae | This study |
pQE1a-Gs1-BH4R | pQE1a-Gs1 derivative; coexpression of GsPheH1 gene and PCD–DHPR operon | This study |
pCDF1b | Protein expression vector; T7 promoter, lacI, CloDF13 ori, SmR | Merck |
pCF1s-Red | pCDF derivative; protein expression vector; tac promoter, lacI, DsRed-monomer gene | This study |
pCF1s-TDC-TYO | pCF1s derivative; expression of TDC and TYO genes as an operon in this order | This study |
pCF1s-TDC-TYO-BH4R | pCF1s-TDC-TYO derivative; expression of PCD–DHPR operon | This study |
pCF1s-TYO-TDC | pCF1s derivative; expression of TYO and TDC genes as an operon in this order | This study |
pBbS1a-2 | Expression of TYO and TDC genes as an operon, trc promoter, lacI, SC101 ori, ApR | [12] |
pBbS1a-3 | Expression of DDC and TYO genes as an operon, trc promoter, lacI, SC101 ori, ApR | [13] |
pBbE1k-3 | Expression of TyrH, DHPR, and PCD genes as an operon, trc promoter, ColE1 ori, KmR | [13] |