Table 2 Strains used in this study
Strains | Description | Sources |
|---|---|---|
Bacillus sp. INT005 | FERM P-18327, a source of BsPheH gene | NITE, [26] |
Cupriavidus necator H16 | ATCC 17699, a source of CnPheH gene | ATCC |
Gulbenkiania sp. SG4523 | NBRC 113456, a source of GsPheH1 and GsPheH2 genes | NITE |
Pseudomonas putida KT2440 | NBRC 100650, a source of PpPheH gene | NITE |
Xanthomonas oryzae | MAFF 311018, a source of XoPheH gene | NARO |
Escherichia coli JM109 | endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB), recA1, F’[traD36, proAB+, lacIq, lacZΔM15] | Nippon Gene |
Escherichia coli BW25113 | Wild type; rrnB3 ∆lacZ4787 hsdR514 ∆(araBAD)567 ∆(rhaBAD)568 rph-1 | NIG |
Y0Km | BW25113 derivative, ∆tyrA with KmR-marker, ∆feaB-tynA without KmR-marker | This study |
Y0 | Y0Km derivative, ∆tyrA without KmR-marker | This study |
YBR | Y0 harboring pSTV-BH4R | This study |
PGs | Y0 harboring pQE1a-Gs1-BH4R | This study |
GsBR1 | Y0Km derivative, Tyr-producing gene cassette integrated at tyrA-knockout region | This study |
GsBR2 | GsBR1 derivative, Tyr-producing gene cassette integrated at feaB–tynA-knockout region | This study |
GsBR2∆aroD | GsBR2 derivative, ∆aroD with KmR-marker | This study |
GsBR3 | GsBR2∆aroD derivative, Tyr-producing gene cassette integrated downstream of aroD locus | This study |
GsBR3∆cysE | GsBR3 derivative, ∆cysE with KmR-marker | This study |
GsBR4 | GsBR3∆cysE derivative, Tyr-producing gene cassette integrated downstream of cysE locus | This study |
GsBR4∆serA | GsBR4 derivative, ∆serA with KmR-marker | This study |
GsBR5 | GsBR4∆serA derivative, Tyr-producing gene cassette integrated downstream of serA locus | This study |