Fig. 6

mRNA levels of xylA and xylB (A) and XylA enzyme activity in crude extracts (B) of C. glutamicum ΔactA::xylAB and the evolved strain C. glutamicum gX. For gene expression analysis by qRT-PCR, cells were grown in CgXII medium with 40 g glucose L⁻¹ or 40 g xylose L⁻¹ (A). Xylose isomerase activity was analyzed in crude extracts of C. glutamicum ΔactA::xylAB and evolved C. glutamicum gX, grown in LB (B). Significance was determined based on a two-sided unpaired Student’s t-test (**p < 0.01). Data represent means of 3 biological replicates with standard deviations