Fig. 8

Pulsed fed-batch cultivation of C. glutamicum ΔfasR cg2692_TTG CgLP12::(P_tac-pntAB-T_rrnB) gX (pEKEx2-maqu2220) with wheat straw hydrolysate. Fed-batch cultivations were conducted in a 1-L BioFlo120^® bioreactor system with an initial batch volume of 0.5 L in CgXII_mod medium supplemented with 195 µM PCA. The carbon source during the batch phase was provided by hydrolysate, normalized to a concentration of 40 g glucose L⁻¹. 30 mL pulses (P) of a 350 g glucose L⁻¹ hydrolysate stock solution were added three times upon a sharp increase of the DO signal. Shown process data are representative of three bioreactor cultivations using the same process conditions. Act acetate, DO dissolved oxygen, Glc glucose, X biomass, Xyl xylose