Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate

Table 1 Growth and production of C. glutamicum gX, Dpa1_gX, MePhe5_gX, and GRLys1ΔsugRΔldhA*_gX with xylose as sole or combined carbon source

Strain	C-source	Biomass, g L⁻¹	µ_max, h⁻¹	Product	Product titer, g L⁻¹	Product yield, g g⁻¹
gX	xylose	n.o.*	n.o.*	l-glutamate	4.0 ± 0.4	0.20 ± 0.02
gX	mix	n.o.*	n.o.*	l-glutamate	3.7 ± 0.2	0.18 ± 0.01
Dpa1_gX	xylose	0.8 ± 0.1	0.07 ± 0.00	DPA	0.5 ± 0.0	0.03 ± 0.00
Dpa1_gX	mix	1.7 ± 0.4	0.05 ± 0.00	DPA	0.7 ± 0.0	0.03 ± 0.00
MePhe5*_gX	xylose	2.0 ± 0.1	0.07 ± 0.00	NMePhe	1.1 ± 0.1	0.06 ± 0.01
MePhe5*_gX	mix	2.9 ± 0.5	0.04 ± 0.00	NMePhe	1.2 ± 0.0	0.06 ± 0.00
GRLys1 ΔsugRΔldhA_gX	xylose	1.1 ± 0.1	0.05 ± 0.00	l-lysine	6.1 ± 0.9	0.30 ± 0.04
GRLys1 ΔsugRΔldhA_gX	mix	2.5 ± 0.2	0.12 ± 0.01	l-lysine	8.9 ± 0.6	0.45 ± 0.03

Cultivations were performed in 500-mL shaking flasks or in Duetz plates (MePhe5*_gX) with CgXII medium supplemented with 20 g xylose L⁻¹ or 15 g glucose L⁻¹ and 5 g xylose L⁻¹ as carbon sources for 48 h (l-glutamate) or 72 h (DPA, NMePhe, l-lysine). Data represent means of 3 biological replicates with standard deviations
*n.o. = no growth observed after ciprofloxacin addition

ISSN: 2731-3654