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Table 3 KPI of a pulsed fed-batch process with C. glutamicum ΔfasR cg2692TTG CgLP12::(Ptac-pntAB-TrrnB) gX (pEKEx2-maqu2220) with wheat straw hydrolysate

From: Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate

Phase

KPI

Batch phase

QP|g L−1 h−1

0.049 ± 0.001

YP/S|Cmol Cmol−1

0.038 ± 0.002

Fed-batch phase

QP|g L−1 h−1

0.227 ± 0.028

YP/S|Cmol Cmol−1

0.065 ± 0.008

Total

QP|g L−1 h−1

0.109 ± 0.005

YP/S|Cmol Cmol−1

0.054 ± 0.005

CDWmax|gX L−1

37.4 ± 1.1

cFAL,max|g L−1

2.45 ± 0.09

  1. The carbon source during the batch phase was provided by hydrolysate, normalized to a concentration of 40 g glucose L−1. 30-mL pulses of a 350 g glucose L−1 hydrolysate stock solution were added three times upon a sharp increase of the DO signal. Data represent means of 3 biological replicates ± standard deviations