Fig. 5

The expression levels of strain YQ-7 feeding on both glucose and malonate were determined relative to control (without malonate) for several genes. A The main carbon metabolic network investigated in transcription analysis. Carbon metabolic flux was switched from fatty acid synthesis to β-caryophyllene synthesis mediated by malonic acid assimilation. The mRNA levels of HXT7, HXK1 and ACC1 genes decreased upon the addition of malonate (blue arrows and blue gene names). The mRNA levels of MCT1 genes boosted considerably after adding malonate (red arrow and red gene name). The profile of other genes expression levels showed no obvious difference with or without malonate (black arrows and black gene names). B Genes related to MVA route. C Genes related to malonic acid metabolic pathway. D Genes related to glyoxylate cycle. E Genes related to glucose metabolism pathway. F Genes related to fatty acids metabolism pathways and other routes. The error bars correspond to standard deviation (n = 3). ICL1, isocitrate lyase; MLS1, malate synthase; FBP1, fructose-1,6-bisphosphatase; MCT1, malonyl-CoA:acyl-carrier-protein transferase