Fig. 1
Evaluating the effect on the dysfunction of sugar transporters for cellulase production. a Diagram of the promoter replace cassette. The native promoter of each sugar transporter was replaced by the copper-controlled Ptcu promoter. b Analysis of cellulase production of Rut-C30, ptcucrt1, ptcustp1, ptcu46819, ptcu69026, ptcu77517, ptcu106556, ptcustr2, ptcu55077, ptcu62380, ptcu65493 in Avicel inducing medium. 20 μM CuSO4 was added to repress the expression of the desired sugar transporters. All the transformants and parent strain were precultured in SDB for mycelia accumulation and transferred to Avicel inducing medium for 96 h. The filter paper activity (FPase) was used to represent the cellulase production and normalized to the intracellular protein content, which referred to the mycelia accumulation on Avicel medium. Values are presented as the mean with the standard deviation from three biological replicates (three flasks). Statistical significance was calculated through Student’s t test. p ≥ 0.5 was indicated as n.s. ** represents p < 0.01