Fig. 3

Subcellular distribution of TRE77517GFP in T. reesei. a C-terminal GFP fused TRE77517 was expressed under the control of strong constitutive promoter Ptef in Rut-C30, resulting in strain ptef77517GFP. ptef77517GFP was cultured in MM medium plus 2 g/L tryptone for 18 h when glucose and lactose was used as carbon source, and for 24 h in Avicel medium. Images were taken under a 63 × oil objective. Scale bar represents 5 μm. b Membrane protein extraction from strain Rut-C30, ptef77517, ptef77517GFP and QM6aptef77517GFP were applied for Western blot using an anti-GFP antibody with 1:2000 dilution. Strains were cultured for 18 h in glucose MM medium before membrane protein extraction. Membrane protein extraction was applied as described in methods section. The blot signal of TRE77517GFP was indicated by the arrow. The calculated molecular weight for TRE77517GFP is 522 amino acid + GFP, while the blot signal is higher than expected which might be attributed to post-translational modification