Fig. 4
Sugar transporter-like protein TRE77517 regulates cellulase induction. a Plate assay of the effect of TRE77517 dysfunction on polarized growth on PDA plates. Approximately 104 conidia of Rut-C30, ptef77517a, ptef77517b, Δ77517 and ptcu77517 were inoculated on the PDA plate and cultured at 30 °C for 5 days before images were taken. 20 μM CuSO4 was added as needed. b Cellulase production on MM medium with 1% Avicel as the carbon source. Strains were precultured on glucose MM medium plus 2 g/L tryptone for mycelia accumulation, then equal amounts of mycelia were transferred to a fresh MM medium with 10 g/L Avicel as sole carbon source. The pNPCase activity was analyzed during fermentation. c Mycelia in Avicel medium are represented as intracellular protein content. d Mycelia were transferred to Avicel medium, and samples were taken at 6 h, 12 h and 24 h. The transcription level of the major cellulase gene cel7a, transcription factor xyr1, cellulase response transporter crt1 and tre77517 was measured and normalized to the transcription of sar1. Values are presented as the mean with the standard deviation from three biological replicates. Statistical significance was calculated through Student’s t test. The p ≥ 0.05 means no significant. p < 0.05 was considered as statistical significance and was indicated as *. p < 0.01 was indicated as **. p < 0.001 was indicated as ***. p < 0.0001 was indicated as ****