Fig. 5

Dysfunction of CRT2 (TRE77517) did not affect cellobiose/lactose consumption. Parent strain Rut-C30, ptefcrt2 and Δcrt2 were precultured on glucose MM medium plus 2 g/L tryptone for 48 h. Equal amounts of pre-grown mycelia were transferred to MM medium with 10 g/L lactose (a) or cellobiose (b) as sole carbon sources. Samples were taken at indicated time, the residual sugar, dry biomass and cellulase activity were measured. c Transcription analysis of cellulase gene cel7a, cellobiose/lactose transporter crt1 and transcription factor xyr1 in lactose and cellobiose medium. Samples were taken at 3 h and 6 h after mycelia were transferred to the MM medium with lactose or cellobiose as the sole carbon source. All the values represent the mean ± S.D. of three individual experiments. n.s. not significant (p > 0.05), *p < 0.05, **p < 0.01, Student’s t test