Fig. 6

CRT2 functions in a CRT1-like manner in cellulase induction by activating CRT1 and XYR1. a Analysis of pNPCase activity in Rut-C30, Δcrt1, Δxyr1, ptefcrt2, ptefcrt2Δcrt1 and ptefcrt2Δxyr1 cultured with 1% Avicel for indicated time. Mycelia were collected for transcription analysis after being transferred to Avicel MM medium for 6 h, 12 h. The transcription of cel7a, crt1 and xyr1 were normalized to the transcription of endogenous sar1. b Effect of deletion of crt1 or xyr1 on crt2 expression on Avicel medium. c Transcription of crt2 and crt1 when the major activator XYR1 is overexpressed on Avicel medium. Samples were taken at indicated time and the transcription of sar1 was set as 1. All the values represent the mean ± S.D. of three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Student’s t test