Fig. 7

Activated cellulase induction through overexpressed crt2 was still repressed by CCR. a Effect of dysfunction of CRT2 for the cellulase production in QM6a background on glucose medium. Equal amount of precultured QM6a, QM6aptefcrt2, QM6aΔcrt2 and QM6aΔcre1 were transferred to fresh MM medium with 10 g/L glucose as carbon source. pNPCase activity was measured after mycelia were transferred for 24 h. n.d. not detected. b Transcription of CCR regulator cre1 on Avicel medium. Precultured mycelia were transferred to Avicel MM medium, samples at 6 h, and 12 h were taken and analyzed for transcription level. c Cellulase production by constitutive expression of crt2 on glucose MM medium in CCR-negative strain Rut-C30. Samples were taken at indicated time and analyzed for pNPCase activity (c) and transcription level (d). All the data were presented as the mean value from three biological replicates. p ≥ 0.05 means no significant. p < 0.05 was considered as statistical significance and was indicated as *. p < 0.01 was indicated as **. p < 0.001 was indicated as ***. p < 0.0001 was indicated as ****