Fig. 7From: Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine productionEffect of pH on enzyme activity of (A) BcAlaDH, (B) BsAlaDH, (C) MsAlaDH and (D) GsAlaDH. The enzyme activity was detected using pyruvic acid as a substrate. The following buffers were used: 0.1 M citrate (pH 3.0–7.0), 0.1 M Tris–HCl (pH 7.0–10.0) and 0.1 M glycine- NaOH (pH 10.0–13.0)Back to article page