Table 1 Kinetic parameters of AlaDH in reductive and oxidative reaction
|  | Specific activity (U/mg) | Km (mM) | Vmax (μmol/min) | kcat (s−1) | kcat/Km (s−1 mM−1) |
|---|---|---|---|---|---|
Reductive reaction | |||||
 BsAlaDH | 5.22 ± 0.03 | 2.25 ± 0.40 | 64.70 ± 6.34 | 36.34 ± 0.18 | 16.68 ± 3.42 |
 BcAlaDH | 5.64 ± 0.34 | 1.10 ± 0.15 | 66.91 ± 3.70 | 40.43 ± 2.93 | 37.92 ± 6.76 |
 MsAlaDH | 4.48 ± 0.62 | 1.69 ± 0.22 | 23.69 ± 1.52 | 28.28 ± 4.39 | 17.02 ± 4.00 |
 GsAlaDH | 2.00 ± 0.06 | 0.81 ± 0.25 | 10.70 ± 1.07 | 11.94 ± 0.06 | 16.29 ± 5.81 |
Oxidative reaction | |||||
 BsAlaDH | 92.20 ± 5.94 | 0.07 ± 0.03 | 148.30 ± 2.10 | 103.97 ± 1.77 | 1819.48 ± 10.25 |
 BcAlaDH | 72.54 ± 1.12 | 1.10 ± 0.08 | 537.90 ± 1.15 | 122.33 ± 2.19 | 111.80 ± 9.67 |
 MsAlaDH | 87.50 ± 2.00 | 0.47 ± 0.03 | 48.09 ± .518 | 136.72 ± 1.95 | 292.09 ± 2.06 |
 GsAlaDH | 31.30 ± 0.42 | 0.23 ± 0.03 | 193.50 ± 2.37 | 108.67 ± 2.13 | 480.66 ± 7.22 |