Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production

Gu, Pengfei; Ma, Qianqian; Zhao, Shuo; Li, Qiang; Gao, Juan

doi:10.1186/s13068-023-02373-5

Biotechnology for Biofuels and Bioproducts

Table 3 Effects of different metal ions on AlaDH

From: Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production

Relative activity (%)
Metal ion	1 mM				5 mM
Metal ion	BsAlaDH	BcAlaDH	MsAlaDH	GsAlaDH	BsAlaDH	BcAlaDH	MsAlaDH	GsAlaDH
Control	100.00 ± 0.90	100.00 ± 0.80	100.00 ± 2.20	100.00 ± 1.60	100.00 ± 0.70	100.00 ± 1.20	100.00 ± 0.70	100.00 ± 1.10
Co²⁺	82.60 ± 0.71	50.95 ± 1.20	72.10 ± 1.27	80.80 ± 2.83	26.10 ± 0.28	45.80 ± 0.85	41.65 ± 0.92	54.80 ± 2.40
Cu²⁺	56.65 ± 0.78	34.30 ± 1.13	44.75 ± 0.49	61.80 ± 1.27	5.10 ± 0.14	2.70 ± 1.13	1.50 ± 0.42	6.10 ± 1.84
Fe²⁺	87.45 ± 0.78	31.35 ± 1.06	55.20 ± 0.57	83.20 ± 1.84	53.70 ± 0.71	14.55 ± 0.47	29.30 ± 1.27	51.65 ± 2.19
Ca²⁺	87.80 ± 1.27	77.80 ± 2.12	87.65 ± 0.64	87.80 ± 1.56	64.30 ± 0.71	53.30 ± 1.70	63.85 ± 0.35	63.90 ± 1.56
Mg²⁺	78.05 ± 1.06	38.05 ± 0.21	96.95 ± 0.78	104.65 ± 3.04	32.70 ± 0.57	56.60 ± 0.99	86.05 ± 0.21	101.5 ± 0.85
Mn²⁺	81.95 ± 0.07	98.05 ± 1.63	92.55 ± 0.78	84.60 ± 0.57	39.25 ± 1.20	60.95 ± 0.35	79.60 ± 1.84	55.65 ± 4.03
Zn²⁺	2.15 ± 0.21	16.05 ± 0.07	8.85 ± 0.35	11.25 ± 1.34	0.00 ± 0.00	1.10 ± 0.28	0.05 ± 0.01	1.05 ± 0.21
Na⁺	103.00 ± 0.28	101.35 ± 0.49	96.25 ± 1.77	113.70 ± 1.98	96.45 ± 1.06	97.60 ± 2.40	94.50 ± 1.27	114.00 ± 2.12
K⁺	103.35 ± 1.63	101.55 ± 0.78	100.25 ± 2.69	105.25 ± 1.34	107.60 ± 1.84	108.55 ± 1.20	103.80 ± 0.42	111.10 ± 2.26
EDTA	63.75 ± 1.63	98.70 ± 1.84	95.05 ± 0.49	83.50 ± 2.26	68.55 ± 0.78	84.40 ± 0.14	78.30 ± 0.99	64.00 ± 1.56
Mercaptoethanol	103.40 ± 1.32	104.25 ± 1.32	102.25 ± 1.55	106.10 ± 1.02	104.75 ± 1.66	105.25 ± 2.05	105.50 ± 1.82	107.10 ± 1.35
DTT	96.60 ± 0.71	98.15 ± 1.20	108.80 ± 0.42	115.35 ± 2.19	108.45 ± 0.44	100.15 ± 1.20	125.25 ± 2.19	110.15 ± 1.20

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