Table 4 Strains and plasmids used in this study
Strains | Relevant genotype | Reference |
|---|---|---|
DH5α | F−, φ80dlacZ, ΔM15, Δ(lacZYA-argF), U169, recA1, endA1, hsdR17(rK– mK+), phoA, supE44, λ−, thi–1, gyrA96, relA1 | Lab stock |
BL21(DE3) | F-, ompT, hsdSB (rB-, mB-), gal, dcm, (DE3) | Lab stock |
M-1 | BW25113(ΔpflBΔpoxBΔadhEΔldhA) | Unpublished data |
M-2 | M-1(ΔmgsA) | This study |
M-3 | M-2(ΔfrdBC) | This study |
M-4 | M-3(ΔdadX) | This study |
M-5 | M-4(pQ-1) | This study |
M-6 | M-4(pQ-2) | This study |
M-7 | M-4(pQ-3) | This study |
M-8 | M-4(pQ-4) | This study |
Plasmids | ||
 pET28a | kan, expression plasmid | Lab stock |
 pN-1 | pET28a-Bsald | This study |
 pN-2 | pET28a-Bcald | This study |
 pN-3 | pET28a-Msald | This study |
 pN-4 | pET28a-Gsald | This study |
 pKD3 | bla, FRT-cat-FRT | [43] |
 pKD4 | bla, FRT-kan-FRT | [43] |
 pCP20 | bla and cat, helper plasmid | [45] |
 pTKRed | spc, helper plasmid | [44] |
 pTrc99a | bla, cloning vector | Lab stock |
 pQ-1 | pTrc99a-Bsald | This study |
 pQ-2 | pTrc99a-Bcald | This study |
 pQ-3 | pTrc99a-Msald | This study |
 pQ-4 | pTrc99a-Gsald | This study |