Fig. 2
From: Combinatorial engineering of betalain biosynthesis pathway in yeast Saccharomyces cerevisiae
The scheme of combinatorial engineering of yeast for improved production of betaxanthins. A combinatorial library of DOD and TyH enzymes was assembled in yeast and the population was enriched for high fluorescence over two rounds of FACS and plated. Individual colonies were cultivated in liquid medium in deep-well plates and fluorescence was measured. The “Ref” strain harbors integration of BvCYP76ADW13L and MjDOD in the same strain background as library isolates. The three highest fluorescent isolates from Library 3 (iso2, iso16 and iso21) were genome sequenced. Data shown are mean values ± SDs of biological triplicates