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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons

Fig. 3

Role of Atf1 in the conversion of phytosterols to 4-HBC. A HPLC profiles of atf1-knockout strain (WIIIΔatf1) and its functional complements (Comp-atf1-WIIIΔatf1 and Comp-sal1-WIIIΔatf1) in the conversion of phytosterols to 4-HBC. B Schematic of the catalytic functions of Atf1 and Sal1. C HPLC profiles of strains NwIB-XIIΔatf1 and NwIB-XII in the conversion of phytosterols. D Effects of atf1 deletion during the conversion of phytosterols to C-19 steroids (AD and ADD) in strain NwIB-XII. All assays were performed in triplicate with three independent measurements. Standard deviations of the biological replicates are represented by error bars

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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