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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons

Fig. 4

Effects of KstR3 on the conversion of phytosterols to 4-HBC in strain WIII. A The growth curves of strains WIII, WIIIΔkstR3 and WIII-261-kstR3 in the MYC/01 medium containing glycerol. B The growth curves of strains WIII, WIIIΔkstR3 and WIII-261-kstR3 in the MM medium with 1 g/L phytosterols as the sole carbon source. C 4-HBC production titers of strains WIII, WIIIΔkstR3, and WIII-261-kstR3. D HPLC profiles of the phytosterols transformation by strains WIII, WIIIΔkstR3, and WIII-261-kstR3. All assays were performed in triplicate with three independent measurements. Standard deviations of the biological replicates are represented by error bars

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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