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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons

Fig. 5

Functional identification of ChsH4. A HPLC profiles of strains WIII, WIIIΔchsH4, and Comp-chsH4-WIIIΔchsH4 in the conversion of phytosterols. B Characteristic genomic distribution of thiolase/aldolase and hydrase genes in sterol-degrading microorganisms. Thiolase/aldolase and hydrase genes are marked in blue arrows, the other genes are marked in black arrows, the length and orientation of arrows represent the length and orientation of genes, the numbers in parentheses indicate the similarity in amino acid sequences. C Schematic of the catalytic mechanism of Sal1 and Shy. D HPLC profiles of strains WIIIΔchsH4, Comp-shy-WIIIΔchsH4 and Comp-sal-shy-WIIIΔchsH4 in the transformation of phytosterols. E Analysis of the conserved domain between ChsH4 and Shy

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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