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Fig. 7 | Biotechnology for Biofuels and Bioproducts

Fig. 7

From: Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons

Fig. 7

Construction of improved strains for the conversion of phytosterols into steroid synthons. A HPLC profiles of strains WIII, WIIIΔCSND, and WIIIΔCSNDΔfadA5 in the transformation of phytosterols. B Production titers of strains WIII, WIIIΔCSND, and WIIIΔCSNDΔfadA5 in the conversion of phytosterols. Effect of individual or combined over-expression of atf1, chsH4, and chsE6 in strain WIIIΔCSNDΔfadA5 on the production of 4-HBC. C Effects of the individual or combined over-expression of atf1, chsH4 and chsE6 in strain WIIIΔCSNDΔfadA5 on the production of 4-HBC. D Effects of the individual or combined deletion of atf1, chsH4, chsE6 or expression of kstR3 in strain NwIB-IΔCSND on the production of 9α-OHAD. All assays were performed in triplicate with three independent measurements. Standard deviations of the biological replicates are represented by error bars

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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