Table 1 Strains and plasmids used in this work
Name | Description | Source |
|---|---|---|
E. coli DH5α | General cloning host | Transgen Biotech |
M. neoaurum ATCC 25795 | M. neoaurum type strain | ATCC |
WIII | Deletion of kshA1, hsd4A, kstD1, kstD2, and kstD3 in M. neoaurum ATCC 25795 | [18] |
NwIB-I | Deletion of kstD1, kstD2, and kstD3 in M. neoaurum ATCC 25795 | [13] |
NwIB-XII | Deletion of kshA1 and kshA2 in M. neoaurum ATCC 25795 | [18] |
WIIIΔfadA5 | Deletion of fadA5 in WIII | This work |
WIIIΔltp2 | Deletion of ltp2 in WIII | This work |
WIIIΔltp3 | Deletion of ltp3 in WIII | This work |
WIIIΔltp4 | Deletion of ltp4 in WIII | This work |
WIIIΔatf1 | Deletion of atf1 in WIII | This work |
WIIIΔatf2 | Deletion of atf2 in WIII | This work |
NwIB-XIIΔatf1 | Deletion of atf1 deleted in NwIB-XII | This work |
WIIIΔatf1ΔchsH4 | Deletions of atf1 and chsH4 in WIII | This work |
WIIIΔkstR3 | Deletion of kstR3 in WIII | This work |
WIIIΔchsH4 | Deletion of chsH4 in WIII | This work |
WIIIΔchsE6 | Deletion of chsE6 in WIII | This work |
WIIIΔopccR | Deletion of opccR in WIII | This work |
WIIIΔchsE6ΔopccR | Deletions of chsE6 and opccR in WIII | This work |
Comp-atf1-WIIIΔatf1 | atf1 complemented in WIIIΔatf1 | This work |
Comp-sal1-WIIIΔatf1 | sal1 complemented in WIIIΔatf1 | This work |
Comp-chsH4-WIIIΔchsH4 | chsH4 complemented in WIIIΔchsH4 | This work |
Comp-shy-WIIIΔchsH4 | shy complemented in WIIIΔchsH4 | This work |
Comp-sal1-shy-WIIIΔchsH4 | sal1 and shy complemented in WIIIΔchsH4 | This work |
Comp-sal1-WIIIΔchsH4 | sal1 complemented in WIIIΔchsH4 | This work |
Comp-atf1-WIIIΔchsH4 | atf1 complemented in WIIIΔchsH4 | This work |
WIII-261-kstR3 | kstR3 over-expressed strain of WIII | This work |
WIIIΔCSND | Deletion of CSND gene cluster in WIII | This work |
WIIIΔCSNDΔfadA5 | Deletion of fadA5 and CSND gene cluster in WIII | This work |
WIIIΔCSNDΔfadA5-I | atf1 over-expressed strain of WIIIΔCSNDΔfadA5 | This work |
WIIIΔCSNDΔfadA5-II | chsH4 over-expressed strain of WIIIΔCSNDΔfadA5 | This work |
WIIIΔCSNDΔfadA5-III | chsE6 over-expressed strain of WIIIΔCSNDΔfadA5 | This work |
WIIIΔCSNDΔfadA5-IV | atf1 and chsH4 co-overexpressed strain of WIIIΔCSNDΔfadA5 | This work |
NwIB-IΔCSND | Deletion of CSND gene cluster in NwIB-I | This work |
NwIB-IΔCSND-I | kstR3 over-expressed strain of NwIB-IΔCSND | This work |
NwIB-IΔCSND-II | Deletion of atf1 in NwIB-IΔCSND | This work |
NwIB-IΔCSND-III | Deletion of chsH4 in NwIB-IΔCSND | This work |
NwIB-IΔCSND-IV | Deletion of chsE6 in NwIB-IΔCSND | This work |
NwIB-IΔCSND-V | Over-expression of kstR3 and deletion of atf1 and chsH4 in NwIB-IΔCSND | This work |