Fig. 4

Analysis of mutations. Panel A shows the count (frequency) of strains with mutations at each locus. Eleven strains were analyzed for this analysis, consisting of the final isolates or populations for each of the three lineages. These strains include LL1787, LL1806, LL1789, LL1727, LL1728, LL1729, LL1730, LL1792, LL1790, LL1791, LL1805. Mutations occurring in three or more strains are described in panel B. Mutations affecting a coding sequence are described by the one-letter amino acid abbreviation for the native residue, the amino acid position, and the mutated residue. Abbreviations include stop: stop codon, fs: frameshift, prom: mutation in promoter region, Tn: transposon insertion. Mutations are color-coded to indicate expected function (see key in figure). Additional mutations from other published work describing ethanol adaptation of C. thermocellum is included for context [6, 7, 16]