Fig. 7From: Ethanol tolerance in engineered strains of Clostridium thermocellumALDH and ADH activity was measured in cell extracts with both NADH and NADPH as cofactors. Enzyme assays were performed in a 60 µl volume in a 384 well plate. Error bars represent one standard deviation, n ≥ 3. Strain LL1592 was constructed with a T. saccharolyticum AdhE containing a G544D mutation, which all descendant strains inheritedBack to article page