Table 2 Strains used in this work

M1442

Thermoanaerobacterium saccharolyticum engineered for production of ethanol at high yield and titer. Also known as strain LL1049

SRP052455

[8]

LL1004

WT C. thermocellum DSM 1313 from DSMZ culture collection

NCBI reference sequence NC_017304.1

LL1570

C. thermocellum expressing a heterologous ethanol production pathway from T. saccharolyticum

∆hpt ΔreIII P2638::adhA(Tsc) nfnAB(Tsc) adhEG544D(Tsc) Clo1313_2637::Ptsc0046-pforA(Tsc)-ferredoxin(Tsc) ∆pfor1 ∆pfor4 ∆pfor3 ∆pfor2 ∆pfor5

SRX4014213

[3]

LL1590

LL1592 ∆adhE

SRX5290158

(This work)

LL1592

LL1570 ∆ldh

SRX5290154

(This work)

LL1679

LL1592 ∆ldh::Ss_gapN

SRX20750948

(This work)

LL1707

LL1592 ∆ldh::Tsc_gapDH

SRX9406543

(This work)

LL1731

LL1679 after adaptation in the presence of added ethanol (population)

SRX9409525

(This work)

LL1732

LL1707 after adaptation in the presence of added ethanol (population)

SRX9409524

(This work)

LL1733

LL1592 after adaptation in the presence of added ethanol (population)

SRX9409526

(This work)

LL1787

Single colony isolate from LL1731 population

SRX20750950

(This work)

LL1806

Single colony isolate from LL1732 population

SRX20750956

(This work)

LL1789

Single colony isolate from LL1733 population

SRX20750951

(This work)

LL1727

LL1592 after adaptation in the presence of added ethanol (population)

SRX9642141

(This work)

LL1728

LL1592 after adaptation in the presence of added ethanol (population)

SRX9642195

(This work)

LL1729

LL1592 after adaptation in the presence of added ethanol (population)

SRX9642247

(This work)

LL1730

LL1592 after adaptation in the presence of added ethanol (population)

SRX9642241

(This work)

LL1734

LL1592 after adaptation in the presence of added ethanol (population)

SRX20750957

(This work)

LL1735

LL1592 after adaptation in the presence of added ethanol (population)

SRX9642242

(This work)

LL1736

LL1592 after adaptation in the presence of added ethanol (population)

SRX20750958

(This work)

LL1737

LL1592 after adaptation in the presence of added ethanol (population)

SRX20750959

(This work)

LL1761

LL1734 population after additional serial transfers (population)

SRX20750961

(This work)

LL1759

LL1735 population after additional serial transfers (population)

SRX20750964

(This work)

LL1760

LL1736 population after additional serial transfers (population)

SRX20750962

(This work)

LL1758

LL1737 population after additional serial transfers (population)

SRX20750960

(This work)

LL1792

Single colony isolate from population LL1761

SRX20750954

(This work)

LL1790

Single colony isolate from population LL1759

SRX20750952

(This work)

LL1791

Single colony isolate from population LL1760

SRX20750953

(This work)

LL1805

Single colony isolate from population LL1758

SRX20750955

(This work)