Fig. 1

Sequence relationship of 5,328 AA9 catalytic modules. A Sequence similarity network (SSN) created with SSNpipe [58] and displayed in Cytoscape with yFiles Organic layout (Shannon et al. 2003a). Each node corresponds to one of the curated 5328 catalytic modules used as an input to build the SSN. Edges represent an alignment bit-score threshold of 250 that clusters the sequences into subgroups. AA9 members whose regioselectivity is available are colored in blue, yellow and red for C1, C4 and C1/C4 oxidizing enzymes, respectively (functional annotations were obtained from the CAZy database [20]). Sequences expressed in Aspergillus niger are colored in black. B Maximum-likelihood phylogenetic tree. 10 representative sequences for each subgroup defined by the sequence similarity network in A. Bootstrap values based on 100 replicates are shown