Fig. 2

HPAEC-PAD analysis of LPMO regioselectivity towards three cellulosic substrates. Chromatograms over the retention times of 7.5–35 min. Native and C1-oxidized cello-oligosaccharide standard peaks are annotated. All activity assays were conducted using 5 µM of enzyme, 0.1% PASC (A), 1% Avicel (B) or 1% SA-Avicel (C) and 1 mM ascorbic acid for 16 h at 50 °C. The negative controls (substrate + electron donor) contained all assay components except enzyme