Fig. 4

Time-course release of soluble oxidized products for A MfLPMO9A, B MsLPMO9B, C DcLPMO9A, and D MytLPMO9A. For each panel, 5 µM LPMOs were incubated at 50 °C for 24 h with 1 mM of either ascorbic acid (black lines), gallic acid (red lines) or cysteine (blue lines) with PASC (squares), Avicel (circles) or SA-Avicel (triangles). For each time point, T. reesei cellulase cocktail was used to convert all C1-oxidized products into cellobionic acid and quantified as the total C1-oxidized ends generated (µM). Total oxidized ends were obtained by quantifying cellobionic acid by HPAEC-PAD against a standard curve. Every point is the average of three independent assays measured individually by HPAEC-PAD, with error bars indicating the standard error of the mean